Volume 59; Issue 1

Main
Methods
Volume 59; Issue 1

Methods

Volume 59; Issue 1

1

Evaluation of qPCR curve analysis methods for reliable biomarker discovery: Bias, resolution, precision, and implications

Ruijter, Jan M., Pfaffl, Michael W., Zhao, Sheng, Spiess, Andrej N., Boggy, Gregory, Blom, Jochen, Rutledge, Robert G., Sisti, Davide, Lievens, Antoon, De Preter, Katleen, Derveaux, Stefaan, Hellemans

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 1.28 MB

english, 2013

2

SPIDIA-RNA: First external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses

Pazzagli, M., Malentacchi, F., Simi, L., Orlando, C., Wyrich, R., Günther, K., Hartmann, C.C., Verderio, P., Pizzamiglio, S., Ciniselli, C.M., Tichopad, A., Kubista, M., Gelmini, S.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 970 KB

english, 2013

3

Transcriptional profiling to address molecular determinants of endometrial receptivity – Lessons from studies in livestock species

Ulbrich, Susanne E., Groebner, Anna E., Bauersachs, Stefan

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 597 KB

english, 2013

4

Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis

Devonshire, Alison S., Sanders, Rebecca, Wilkes, Timothy M., Taylor, Martin S., Foy, Carole A., Huggett, Jim F.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 685 KB

english, 2013

5

Detecting and visualizing gene fusions

Supper, Jochen, Gugenmus, Claudia, Wollnik, Johannes, Drueke, Tanja, Scherf, Matthias, Hahn, Alexander, Grote, Korbinian, Bretschneider, Nancy, Klocke, Bernward, Zinser, Christian, Cartharius, Kerstin

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 515 KB

english, 2013

6

Transcriptional biomarkers – High throughput screening, quantitative verification, and bioinformatical validation methods

Riedmaier, Irmgard, Pfaffl, Michael W.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 360 KB

english, 2013

7

Transcriptional biomarkers

Pfaffl, Michael W.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 136 KB

english, 2013

8

Integrated expression profiling of multiple RNA species by real-time PCR

Yerramilli, Subrahmanyam, Shi, Paul, Kreutz, Martin, Qin, James, Winter, Sherry, Lader, Eric

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 405 KB

english, 2013

9

microRNA biomarkers in body fluids of prostate cancer patients

Kuner, Ruprecht, Brase, Jan C., Sültmann, Holger, Wuttig, Daniela

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 507 KB

english, 2013

10

Combinational usage of next generation sequencing and qPCR for the analysis of tumor samples

Loewe, Robert P.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 1003 KB

english, 2013

11

Gene expression analysis of normal and colorectal cancer tissue samples from fresh frozen and matched formalin-fixed, paraffin-embedded (FFPE) specimens after manual and automated RNA isolation

Kalmar, Alexandra, Wichmann, Barnabás, Galamb, Orsolya, Spisák, Sándor, Tóth, Kinga, Leiszter, Katalin, Tulassay, Zsolt, Molnár, Béla

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 437 KB

english, 2013

12

RT-qPCR work-flow for single-cell data analysis

Ståhlberg, Anders, Rusnakova, Vendula, Forootan, Amin, Anderova, Miroslava, Kubista, Mikael

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 1.72 MB

english, 2013

13

Digital PCR strategies in the development and analysis of molecular biomarkers for personalized medicine

Day, Elizabeth, Dear, Paul H., McCaughan, Frank

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 447 KB

english, 2013

14

Droplet Digital™ PCR quantitation of HER2 expression in FFPE breast cancer samples

Heredia, Nicholas J., Belgrader, Phillip, Wang, Shenglong, Koehler, Ryan, Regan, Jack, Cosman, Angela M., Saxonov, Serge, Hindson, Benjamin, Tanner, Stephanie C., Brown, Alexandra S., Karlin-Neumann,

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 689 KB

english, 2013

15

Assessing sample and miRNA profile quality in serum and plasma or other biofluids

Blondal, Thorarinn, Jensby Nielsen, Søren, Baker, Adam, Andreasen, Ditte, Mouritzen, Peter, Wrang Teilum, Maria, Dahlsveen, Ina K.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 408 KB

english, 2013

16

Gene expression analysis in biomarker research and early drug development using function tested reverse transcription quantitative real-time PCR assays

Lohmann, Sabine, Herold, Andrea, Bergauer, Tobias, Belousov, Anton, Betzl, Gisela, Demario, Mark, Dietrich, Manuel, Luistro, Leopoldo, Poignée-Heger, Manuela, Schostack, Kathy, Simcox, Mary, Walch, He

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 1.35 MB

english, 2013

17

Improving biological relevancy of transcriptional biomarkers experiments by applying the MIQE guidelines to pre-clinical and clinical trials

Dooms, M., Chango, A., Barbour, E., Pouillart, P., Abdel Nour, A.M.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 302 KB

english, 2013

18

Cover 1

Journal:

Année:

2013

Fichier:

PDF, 668 KB

2013

19

Editorial Board

Journal:

Année:

2013

Fichier:

PDF, 26 KB

2013

20

RNA biomarkers in colorectal cancer

Bustin, Stephen A., Murphy, Jamie

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 650 KB

english, 2013

21

Identifying transcriptional miRNA biomarkers by integrating high-throughput sequencing and real-time PCR data

Rahmann, Sven, Martin, Marcel, Schulte, Johannes H., Köster, Johannes, Marschall, Tobias, Schramm, Alexander

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 834 KB

english, 2013

22

Genetic and epigenetic factors in regulation of microRNA in colorectal cancers

Vinci, Serena, Gelmini, Stefania, Mancini, Irene, Malentacchi, Francesca, Pazzagli, Mario, Beltrami, Cristina, Pinzani, Pamela, Orlando, Claudio

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 1.35 MB

english, 2013

23

Gene expression analysis of both mRNA and miRNA on the same TaqMan® Array Card

Ferlinz, Astrid, Miller, Coleen, Formosa, Rachel, Lee, Kathleen Y.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 524 KB

english, 2013

24

The challenge of gene expression profiling in heterogeneous clinical samples

Rodrıguez-Gonzalez, F. German, Mustafa, Dana A.M., Mostert, Bianca, Sieuwerts, Anieta M.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 926 KB

english, 2013

25

Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level

Norrman, Karin, Strömbeck, Anna, Semb, Henrik, Ståhlberg, Anders

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 2.26 MB

english, 2013

26

Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells

Livak, Kenneth J., Wills, Quin F., Tipping, Alex J., Datta, Krishnalekha, Mittal, Rowena, Goldson, Andrew J., Sexton, Darren W., Holmes, Chris C.

Journal:

Année:

2013

Langue:

english

Fichier:

PDF, 1.02 MB

english, 2013